Analysis Note

ControlHeLa, A431, and HUVECs.

Application

Anti-KRIT-1 Antibody, clone 15 detects level of KRIT-1 & has been published & validated for use in IC, IP & WB.

Research CategoryCell Structure

Research Sub CategoryAdhesion (CAMs)

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Cerebral cavernous malformation (CCM), a disease associated with defective endothelial junctions, results from autosomal dominant CCM1 mutations that cause a loss of KRIT-1 protein function. CCM1 has been identified as the KRIT-1 gene. The protein product, KRIT-1, also known as Krev interaction trapped protein 1, is a microtubule-associated protein involved in endothelial cell-cell and cell-extracellular matrix interactions. KRIT-1 binds to Rap1 that maintains the integrity of endothelial junctions. It is reported that KRIT-1 colocalizes and is physically associated with junctional proteins via its band 4.1/ezrin/radixin/moesin (FERM) domain, (Glading, 2007).

Immunogen

GST-tagged recombinant protein corresponding to human KRIT-1 at and around the FERM domain.

Epitope: FERM domain

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein G Purified

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Format: Purified

Quality

Immunocytochemistry Analysis: 1:500 dilution of this antibody detected KRIT-1 in HeLa, A431, and HUVECs.

Specificity

The antibody recognizes KRIT-1.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~ 84 kDa